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A sample of muscle tissue from each specimen was extracted using an automated Glass Fiber protocol [11].
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DNA from the legs of a single specimen was extracted using the DNeasy Kit (Qiagen, Germany) following the manufacturers protocol.
Genomic DNA from clinical specimens was extracted using a DNeasy kit (Qiagen, Valencia, CA).
DNA from Museum specimens was extracted using a Qiagen QIAamp DNA micro kit according to the manufacturers instructions.
DNA from tumour and surgical margin specimens was extracted using the QIAamp Tissue kit (Qiagen, Courtaboeuf, France).
Total RNA from healthy specimens and 119 neoplastic specimens were extracted using Trizol® according to the manufacturer's instructions and in combination with Qiagen RNAeasy Mini Kit (Qiagen).
Following microdissection of tumor cells from sections of tumor specimens, DNA was extracted using the Easy-DNA™ kit (Invitrogen).
DNA was extracted using a standard phenol-chloroform protocol from voucher specimen tissue.
DNA from tissues and swab specimens was extracted bu using the DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany).
The concentration of the protein extracted from each specimen was quantified using the BCA protein assay kit.
Specimens were extracted from the field laid asphalt mixes.
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