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Five micrograms of total RNA from each sample were treated with 0.5 U of DNAse I, (Promega Corp., Madison, WI) for 30 min at 37°C.
Briefly, 600 μg proteins from each sample were treated with DTT (100 μl/1 μl 1 M DTT), alkylated with IAA (100 μl/2 μl 1 M IAA) and ultrafiltered with digestion buffer (50 mM ammonium bicarbonate).
Total RNA was extracted following the protocol standardized by Salzman et al. (1999) [ 92]. 2 μg of total RNA from each sample were treated with DNAse I (Promega), and the treated samples were analyzed in agarose gels to ensure absence of DNA and no degradation.
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In brief, total RNA (18 μg) from each sample was treated with DNase I (Invitrogen).
The separation of metal ions from the matrix of water sample was enhanced when the sample was treated with precipitating ligand.
The sample was treated with antibiotics (C.
All RNA preparations from both cell and tissue samples were treated with DNAase.
Evolved phage populations were isolated from microcosms by centrifugation, and samples were treated with chloroform.
To liberate galangin from its glucuronides, some rat plasma samples were treated with β-glucuronidase.
All samples were treated with DNAse I to ensure they were free from DNA contamination (Turbo DNA-free, Ambion).
The samples were treated with HPPIB.
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