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Sperm from each sample were stained by modified Papanicolaou stain and evaluated manually for normal morphology.
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The nanocable samples were stained by phosphotungstic acid before TEM observation.
The samples were stained by the method of EnVision (enhance labeled polymer system).
These samples were stained by IF.
Samples were stained by antibodies against CD44 and CD133.
Each sample was retrieved from formalin-fixed, paraffin-embedded tissue blocks from which sections were stained by using FIPV 3 70 antibody (Custom Monoclonals, Sacramento, CA, USA).
Epithelial cells isolated from nine independent, normal human breast samples were stained with Hoechst and analysed by FACS.
After 4 h, the mixed samples were stained with PI and analyzed by FACS.
Samples were stained for γH2AX as described previously by Nakamura et al. [25].
Subsequently, samples were stained using Perl's iron stain and corresponding fields of view were evaluated by light microscopy.
The samples were stained with calcofluor white.
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