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After sonicating for 15 s (×2) and centrifuging at 14,000 RPM for 15 min, the protein concentration in the supernatant was determined by the Bradford assay (Bio-Rad, Mississauga, Ont). 25 ug of protein from each sample was resolved using 10% SDS-PAGE before transferring to nitrocellulose membranes (Bio-Rad).
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Samples were resolved using 4 20% SDS-PAGE gels and then dried and analyzed by autoradiography.
Samples were resolved using SDS-PAGE (12.5% gels) and transferred to nitrocellulose filters for Western blotting by transverse electrophoresis.
Samples were resolved using SDS-PAGE followed by Coomassie staining.
The samples were resolved using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometer (Sequenom, San Diego, CA).
Samples were resolved using a 10% polyacrylamide gel and transferred to a Millipore Immobilon-FL polyvinylidene difluoride membranes (Millipore).
Protein quantity was determined using the Bradford protein assay (Bio-Rad, Hercules, CA, USA), and 50 μg of total protein from each sample was resolved by SDS PAGE gels and blotted onto polyvinylidene difluoride membranes (Nihon Millipore Ltd ,Tokyo, Japan).
An equivalent amount of protein from each sample was resolved by 12% SDS PAGE and transferred to a nitrocellulose membrane.
A 20- μg sample of the total protein was resolved using 12% SDS-PAGE and transferred onto PVDF membranes.
An aliquot of each sample was resolved by SDS-PAGE and transferred using standard semi-dry conditions.
13 μl of each sample was resolved by SDS-PAGE prior to staining using Instant Blue SafeStain (Expedeon).
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