Sentence examples for from each sample was performed using from inspiring English sources

Reverse transcription of 600 ng total RNA from each sample was performed using Superscript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) in combination with random primers (Invitrogen, Carlsbad, CA) and oligo (DT 23 (Sigma).

The total RNA extraction from the pretreated stool sample was performed using commercial RNA extraction kit.

Multivariate analysis on the population sample was performed using JMP 9 from SAS (Cary, NC).

DNA extraction from blood samples was performed using the QIAamp DNA Blood Mini Kit from Qiagen.

RT-PCR amplification of template RNA from different samples was performed using ExTaq kit (TaKaRa) using manufacturer's protocol.

DNA extraction from blood samples was performed using the FlexiGene kit (Qiagen), from FFPE samples, after manual macrodissection, using the Ambion RecoverAll kit (Applied Biosystems) and, from frozen samples, using the Nucleospin® Tissue kit (Macherey-Nagel EURL).

DNA extraction from frozen blood samples was performed using the Puregene Kit (Qiagen, Hilden, Germany).

Bisulfite treatment of genomic DNA from APL or AML samples was performed using the EpiTect bisulphite kit (Qiagen, Hilden, Germany).

Area sampling was performed using the Cavalieri estimator probe.

Sampling was performed using standard data collection.

Isolation and cultivation of Mycoplasma strains from tissue samples were performed using standard methodology [ 21].