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10 50 mg tissue from each sample was homogenised using the TissueLyser tissue disruptor (QIAGEN, Germany) for 2× 30 seconds at 20,000 rpm.
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The sample was homogenised using a Q1A shredder (Qiagen® Ltd).
Briefly, 1500 μl of Trizol reagent was added to each 2 ml tube and samples were homogenised using a rotor shaker.
After transfer to ice-cold RNeasy RLT lysis buffer (Qiagen, Courtaboeuf, France), LT tissue samples were homogenised using a Precellys tissue homogeniser (Bertin Technologie, Montigny-le-Bretonneux, France).
Samples were homogenised using a T8 Ultra-Turrax homogeniser (IKA Works, Inc., Wilmington, NC, USA).
Tissue samples were homogenised using the stainless steel-beads and Qiagen-TissueLyser II homogeniser method accordingly to the manufacturer's instructions (Qiagen, Hilden, Germany).
Swab samples were homogenised using a vortex for 1 min, and viral transport medium aliquots were frozen at −80 °C.
Tumour samples were homogenised using a mortar and pestle.
Tissue samples were homogenised using a Mikro-Dismembrator (Braun Biotech T1 International, Melsungen, Germany).
Tissue samples were homogenised using a Mikro-Dismembrator U (Braun Biotech Intl., Melsungen, Germany) to yield a fine powder.
Frozen samples were homogenised using a high speed grinder (IKA-Werke, Staufen, Germany) and total RNA extracted using a modified version of Lewinsohn's protocol [ 17].
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