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Peptide identification from each sample was done using LTQ Orbitrap XL Thermo Scientificc, Waltham, MA).

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Purification of whole histones from snap-frozen tissue samples was done using sulfuric acid.

Thermo-gravimetric analysis (TGA) of all samples was done using SDT Q600 instrument from TA instrument.

Sampling was done using the NewCAST software from Visiopharm.

Sample size calculation was done using the software PASS 2008.

Finally, a prospective observational study was done using 106 clinical samples obtained from 76 patients in our ICU.

Genomic DNA was obtained from blood samples and the screening for the gene polymorphisms was done using polymerase chain reaction-restriction fragment length polymorphism approach.

The item analysis was done using the data from the multi-condition group (Sample 1).

Library preparation was done using a modified protocol from Illumina with NEBNext DNA sample Prep Reagent kits (NEB).

Because this study was done using waste fluid from therapeutic synovial fluid aspirates, we did not have samples from noninflamed joints.

Sample statistics were done using the Wilcoxon test.

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