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The relative expression level of mature miR-26b from each sample was determined using the 2 −Delta Delta C T) Method [42].
Protein in the supernatant obtained from each sample was determined using standard Lowry method.
RNA quality from each sample was determined using the A260/280 absorbance ratio and by electrophoresis on 1.2% agarose formaldehyde gel.
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Protein concentrations for each sample were determined using bicinchoninic acid (BCA) protein assay kit (Thermo Scientific).
Finally, absorbance values were calculated for standards and samples and the concentration of each sample was determined using the standard curve.
The supernatant was carefully removed, and concentrations of each sample was determined using a bicinchoninic acid (BCA) assay.
Total mRNA concentrations from each sample were determined using a spectrophotometer (NanoDrop 2000c, Thermo Scientific, Wilmington, DE, USA).
The RNA concentration from FFPE samples was determined using the NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA).
Concentration of reactive amino groups in the modified magnetite samples was determined using the data from thermogravimetric analysis.
Statistical differences between signals from different samples were determined using two-tailed student t-tests.
Gene expression profiles from these samples were determined using high-throughput Affymetrix gene expression microarray chips.
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