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Following the production of labeled RNA we prepared enough hybridization mixture from each preparation to hybridize both a U133A array and a U133 Plus array at the same time.
Around 100 proteins were quantified from each preparation.
Ten images from each preparation were taken.
Nasal turbinate tissues were homogenized in MEM medium and centrifuged at 400 g for 10 min. Serial 10-fold dilutions of supernatants collected from each preparation were inoculated into three 10- to 11-day old embryonated SPAFAS hen's eggs.
One microgram of total RNA from each preparation was labeled with Hy3 using the miRCURY LNA microRNA Power labeling kit (Exiqon) and then hybridized to miRCURY LNA microRNA array v.10.0 (Exiqon).
200 cells from each preparation were randomly selected and each experiment was performed in triplicate cover slips, more than 600 cells of each cell group per experiment were measured.
Similar(31)
Reproducibility is also an issue if the cells are freshly isolated for each preparation from different donors.
The normalized passive resting force and the corresponding diameter were then determined for each preparation from its own length-pressure curve, according to Mulvany and Halpern [ 26].
Values from two replicates of each preparation were measured.
Several overview pictures were taken randomly from different areas of each preparation.
We performed two independent NK cell preparations from four animals, and two independent PCRs from each cell preparation.
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