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Briefly, one gram of soil from each microcosm was extracted by using the UltraClean Microbial DNA Kit (Mo Bio Laboratories, CA).
For every 48 h, 2 μL (1%) of culture from each microcosm was transferred to 198 μL of fresh medium; migration within each metapopulation was then carried out by transferring 1% of each microcosm to the neighboring microcosms.
At every transfer, 2 μL (1%) of culture from each microcosm was transferred to 198 μL of fresh medium, and for each microcosm with phage treatment, approximately 10 ancestral phage particles were introduced to ensure the persistence of the phage; immigration was then carried out immediately by transferring 1% of each source microcosm to the recipient microcosm.
The temperature program to reduce peak tailing included an initial temperature of 35°C for 5 min, temperature increase at a rate of 25°C/min, and final temperature of 150°C for 10 min. DNA was extracted from 5-mL microcosm or 130-mL groundwater samples using the same protocol.
I was extracted from them".
DNA was extracted from adult S. japonicum.
RNA was extracted from sorted cell subsets.
DNA was extracted from blood or hair.
A significant percentage of the oil that's extracted from Saudi Arabia is used internally.
Water transfer between the reservoir and microcosm was accomplished using two Tom Aquatics Aqualifter dosing pumps (Premium Aquatics, Indianapolis, Illinois, USA) mounted to the outer lip of the microcosm; one pump transferred water into and the other withdrew water from the microcosm.
As its name indicates, graphene is extracted from graphite, the material used in pencils.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com