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At postnatal day 20, half of the rats from each maternal group were weaned onto chow or HFD for 15 weeks.
Seeds from each maternal plant were labeled and kept separate.
In total, 100 mature fruits from each maternal tree were collected in October 2011.
One sample from each maternal family was randomly chosen for genotyping (22 28 individuals per population, n = 364).
When enough seed was available, we planted two seeds per container to increase our likelihood of having a plant from each maternal line.
One plant from each maternal family was randomly crossed in a greenhouse at Blandy Experimental Farm (Boyce, VA, USA) to produce a fully outbred base population.
Similar(45)
We examined placentas from heterozygous embryos for each maternal genotype at E13.5.
The proportion of participants from each cohort in each public health centre was added as a separate variable to the model to compensate for the different participant rates from each cohort in each maternal health centre.
For each maternal family from the 15 populations, 1 4 seed were planted into each of the three pots (0.5 L) in April 2004 (n = 3 12 seed per family).
Distance of each maternal residence from all existing (not abandoned) natural gas wells within a 10-mile radius was then computed using spherically adjusted straight line distances.
To adjust for possible between-centre confounders we adjusted for the mean number of participants from the intervention cohort within each maternal health care centre.
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