Elaborate regulation mechanisms result in numerous cDNA and protein isoforms being expressed from each gene of the p53 family.
The reverse transcription polymerase chain reaction products were checked by agarose gel electrophoresis in order to verify that a single band amplification product from each gene was present.
The half set of chromosomes from an egg and the half set from a sperm combine during fertilization, which gives each offspring a copy of one gene from each gene pair of its female parent and a copy of one gene from each gene pair of its male parent.
Multiple clones from each gene were sequenced.
Seven An. quadriannulatus A alleles from each gene were used to calculate inter-specific divergence.
The data from each gene was provided from two independent experiments.
A single isoform from each gene (i.e. the isoform with the greatest number of codons) was used for analyses.
Signal from each gene was normalized to the means of three 106/1K76 replicates (non CQ-treated).
The model of static exon and intron definitions yielding a single mRNA transcript and single protein sequence from each gene has proven overly simplistic.
To identify differentially expressed genes we fit a general linear model with terms for sex and strain to the data from each gene.
A minimum of five independent clones from each gene segment for each virus were sequenced using a commercial sequencing service (GATC Biotech, Constance, Germany).
