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The plasmid DNA from each clone was extracted using a DNA plasmid Miniprep kit (Promega Corporation, Madison, WI, USA) and then analysed by EcoRI restriction digestion.
The plasmid DNA from each clone was extracted using a DNA plasmid Miniprep Kit (Promega, Madison, USA) and sequenced using M13 primers.
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Genomic DNA was extracted from the proposita's EBV cell line and her parents' lymphocytes using standard protocols; genomic DNA from hybrid clones was extracted using DNAzol (MRC Inc., Cincinnati, OH).
Genomic DNA from 6TG/8AG resistant clones was extracted using the PureGene Genomic DNA Purification Kit (Gentra Systems, Minneapolis, MN).
Plasmid DNA from TOPO clones were extracted using the QIAprep Spin Miniprep kit (QIAGEN), fosmid DNA was extracted using the Plasmid Midi Kit (QIAGEN), and DNA from shot-gun libraries were extracted using the DirectPep 96 MiniPrep (QIAGEN).
Plasmids from positive clones were extracted using QIAquick PCR purification kit (Qiagen).
Plasmids from the resulting bacteria clones were extracted using the Plasmid Mini Kit from Qiagen (Hilden, Germany) and the plasmid inserts were sequenced.
RNA from dermis was extracted using Trizol reagent (Invitrogen).
The plasmid DNA from these clones (i.e. colonies) was extracted using a 96-well alkaline lysis method prior to sequencing [ 69].
DNA from the identified BAC clones (including those that were screened) was extracted using Wizard® Plus SV Minipreps DNA Purification System (Promega, Australia).
A total of 32,026 clone cultures with growth at ODs ranging from 0.6 to 1.0 were pooled, pelleted and the DNA was extracted using the standard alkaline lysis method.
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