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The gProcessedSignal from each array was loaded into Partek Genomics Suite (v6.6) and normalized between arrays using quantile normalization.
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A cantilever array was loaded into the fluid chamber and allowed to equilibrate in deionized water.
A cantilever array was loaded into the fluid chamber and allowed to equilibrate in nanopure water.
A cantilever array was loaded into the measurement chamber and allowed to equilibrate in 5% glycerol solution (v/v).
A cantilever array was loaded into the fluid chamber that was subsequently sealed either with Nescofilm or an O-ring and allowed to equilibrate in nanopure water.
The array was loaded into a Corning hybridization chamber and incubated at 42°C for 16 18 hours.
From this, 30 μl was loaded into each well on the gel.
From each sample, ca 0.25 mg was loaded into 10 ml Labco Exetainer® vials.
The sample/master mix for each Megaplex pool (100 μl) was loaded into the array, centrifuged and mechanically sealed with the Applied Biosystems sealer device.
A single-use test cartridge containing the slide array and hybridization reagents was loaded into the Verigene Processor SP, and the assay was started.
The card was then placed in a Taqman array slide sealer, sealed, and the loading ports cut from the card before it was loaded into an AB 7900HT thermal cycler.
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