Sentence examples for from dynabeads from inspiring English sources

Immunoprecipitates were eluted from Dynabeads using glycine-HCl (pH 2) treatment for 10 min and were subsequently neutralized with neutralization buffer (0.5 M Tris HCl, 1.5M NaCl).

Complete Protease Mixture from Roche Applied Science; cycloheximide from Sigma; immobilized 2'/3'-EDA-7-methyl-GTP agarose beads from Jena Bioscience GmbH, Germany; DMEM from Life Technologies Inc.; inactivated fetal calf serum from Invitrogen; Dynabeads® magnetic separation system from Life Technologies Inc.

Messenger RNA was extracted directly from tissue with Dynabeads from the mRNA Direct Kit (Invitrogen) with only one round of bead hybridization.

The enriched cells were detached from the Dynabeads CD4 by incubation for 45 60 minutes at room temperature with 10 µl of DETACHaBEAD (Dynal).

Also, following the first phase of the hereditary cancer study, protocol recommendations were updated to enable PCR amplification directly from the Dynabeads® (Life Technologies Ltd., Paisley, UK).

Finally, the sample was eluted from the Dynabeads® by adding 25 μL of elution buffer and incubating for 10 min at room temperature before pelleting in a magnetic rack.

The washing step using the LiCl washing buffer was skipped and elution of the crosslinked chromatin from the Dynabeads® – antibody conjugate was performed in presence of SDS 0.1%/NaHCO3 (10 mM).

IgG Sepharose was from Amersham, Ni-magnabeads were from Promega and the Dynabeads M280 were from Dynal Biotech.

Briefly, first-strand cDNA was synthesized from oligo dT) Dynabead-captured mRNA using SuperScript VILO cDNA Synthesis Kit (Invitrogen).

Purification of PTH-AGO1-RNA on Ni-NTA Agarose The eluate from IgG-Dynabeads was loaded on 40 μl of Ni-NTA Agarose (QIAGEN) equilibrated with Ni-WB I for 2 hr at 4°C and then Ni-NTA beads were washed with Ni-WB I buffer.

About 400 bacterial clones were picked onto each square agar plate (Bio-Assay dish, Nunc) for colony hybridisation with P-labelled cDNA probe, derived from mRNA extracted by oligo dT 25 Dynabeads (Dynal) from the same muscle type and stage of development as that used for the library construction.