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pBAC-BoHV-4ΔIE1 was stable as observed by the absence of differences among the restriction profile of pBAC-BoHV-4ΔIE1 derived from different passages (Fig. 1c).
One e-mail message that was widely circulated last week combines sentence fragments from different passages in Nostradamus's writings with words that were not his, to create a provocative text whose implication was impossible to mistake: "In the year of the new century and nine months/ From the sky will come a great King of Terror," the e-mail message read.
The data were acquired from three independent experiments (conducted in triplicate) using cells from different passages.
There was no variation between the phenotypes of cells from different passages or freezing thawing cycles.
Cells from different passages of each seeding density were subjected to pluripotency genes (Oct4, Sox2, Nanog, klf4, c-Myc) and PDGFRa gene expression analysis, measurement of absolute telomere length by real-time PCR, and induction of differentiation into osteogenic, adipogenic, and chondrogenic lineages.
Therefore, the present study was designed (1) to investigate the epigenetic changes after prolonged culture in vitro of porcine embryonic germ (EG) cells, including differences in expression levels of both DNA methylation and demethylation-related genes and catalyses of histone modifications, and (2) to assess the efficiency of SCNT using EG cells from different passages.
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Subsets of fish were collected from different passage conditions according to facility guidelines based on US Fish and Wildlife Service fish hatchery management practices, and in accordance with approved animal care and use protocols.
Twenty fish each from different passage scenarios and 10 control fish were collected and euthanized in 250 mg/L tricaine methane sulfonate followed by decapitation prior to excising whole brain tissue.
We mixed equal cell concentrations of a related GFP-marked reference strain and one of several YJB9929 isolates from different passaging time points.
However, the various CM, even when they were derived from the same kind of cells, were produced by different condition, that is, from different passage, culture medium, and culture condition.
The various conditioned media, even when they were derived from same kind of cells, were produced by different condition, that is, from different passage, number of cells, culture medium, and culture condition (Table 2).
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