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However, the HHV of bio-chars from different leaves behaved differently, with the bio-char from wintergreen leaves showing the highest HHV, which reached around 27 MJ/Kg; on the contrary, the HHV of bio-chars from bamboo leaves gave the lowest values, some 20 22 MJ/Kg.
The experimentally determined higher heating values (HHV) of bio-chars from different leaves ranged from 20 to 28 MJ/Kg, interestingly, which are comparable to some coals.
There was a minimum of three independent replicates for each experiment with tissue derived from different leaves.
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For each bacterial strain, cells were recovered from 4 different leaves; two samples were collected from each specimen leaf.
Each value is the mean from 6 different leaves of 6 different plants measured from three sunny days.
Each value is the means from 6 different leaves of 6 different plants.
SD from three different leaves with three points on one leaf of same line has been represented.
mCherry fluorescence in 60 cells from three different leaves was classified into cytoplasmic and nuclear localization or exclusive nuclear localization.
For each of three replicates, we tested a leaf sample of 100 mg consisting of pieces (about 5 mg each) from 20 different leaves.
After the specified time period leaves were cut into halves and the control and treated halves from 4 different leaves were pooled together, immediately frozen in liquid nitrogen, weighed and kept at −80 °C until further analysis.
Total RNA from three different leaves were used in qPCR reactions as independent biological replicates, and three technical replicates for each biological sample were considered for statistical T-tests to determine the significant changes of gene expression across the treatments.
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