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Variation in gene expression may result from changes in the sequence of regulatory elements, such as single nucleotide polymorphisms (SNPs), and recent surveys indicates that this phenomenon is widespread through the genome and tissues [3], [12], [13], [14].
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Because mitochondrial DNA is passed down from mother to daughter without combining with male DNA, any changes in the sequence of nucleotides are due to mutations.
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Sequence variants are inferred from changes in the melting transition of the PCR product as, depending on their GC content, length or sequence, different PCR products have different melting temperatures [12], whether mutated sequences are known or not.
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His client had play a minor role in the sequence of events but during the inquest, the case changed.
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