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miRNA and mRNA expression data from cell lines was produced using Agilent Tehcnologies' (Santa Clara, CA) miRNA microarray system (version 1 array containing 470 human and 64 viral miRNAs) 8 and Whole Human Genome Kit (4 × 44k) (Agilent Tehcnologies, Santa Clara, CA), respectively.
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Stable YFP-paxillin expressing REF-52 cell line was produced using retroviral infection.
Stably infected cells lines were produced using lentiviruses.
In 2005, a human beta cell line NAKT-15 was produced using human adult islets as starting material [20].
The EBV-positive cell lines were produced by artificial EBV infections using marker selections.
Donor fibroblast cell lines were produced from biopsies taken from a male and a female adult sheep and cumulus cell lines were harvested from the same ewe.
Roughly one iPC cell line was produced for every 5000 cells the researchers treated using the technique, an efficiency that enabled them to produce several cell lines from each experiment.
The HPV16+ 2A3 cell line was produced by stably transfecting the FaDu cell line from the American Type Culture Collection with the LXSN 16E6/E7 as described in Harris et al (2011).
An identical band was produced using RNA from the androgen sensitive prostate cancer cell line LNCAP, (not shown).
rFIXFc was produced using a process free of human- and animal-derived raw materials and a host cell line derived from human embryonic kidney (HEK) 293H cells.
Cells passage was performed every 3 4 days and suspensions of each cell line were produced from confluent cultures using 10× TrypLE™ (gibco® by life technologiesTM, USA).
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