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LRP5 gene expression was higher in mature adipocytes compared with SVCs from both depots.
However, adipocytes from both depots display common pathways and generally similar alterations in gene expression in response to aging.
Because both the absolute amount of visceral fat and the visceral-to-subcutaneous fat ratio are determinants of the clinical metabolic characteristics of obese individuals, a lower ratio at baseline probably raises the chances of clinical improvement because the ratio remains fairly constant due to proportional fat loss from both depots.
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In our study, we included samples from both fat depots and we identified numerous genes differentially expressed in VAT and SAT.
Furthermore, DPP4 expression significantly increased in both depots with increasing BMI from lean to obese subgroups (Fig. 1 C ).
Thus, all subjects lost a comparable significant amount of fat from both abdominal fat depots yet maintained a constant ratio between these depots.
Also, the release of RANTES from both of these depots correlated significantly with omental MCP-1 (for subcutaneous RANTES r = 0.66, P = 0.02; for omental RANTES r = 0.80, P = 0.001) and omental IL-6 (for subcutaneous RANTES r = 0.62, P = 0.02; for omental RANTES r = 0.55, P = 0.04).
This allows the comparison of adipocytes from depots where both large and small numbers of precursors can be obtained, using fewer mice.
It has been extensively shown that distinct WAT depots from both mice and humans have different metabolic functions.
These data indicate that there are large differences in immune cell composition between different WAT depots from both lean and obese mice.
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