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The images in each row are ordered from apex (left) to basis (right).
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Bract tissues were removed from the apex leaving the leaves and reproductive tissue, which was fixed in FAA (50% ethanol, 5% glacial acetic Acid, 3.7% formaldehyde) under vacuum (20 In. Hg) at room temperature.
The remainder of the left ventricular wall, including the apex, is left free.
For microscopic observations, the samples of S. flavescens roots (5 10 cm from tap root base), stems (the fourth or fifth node from shoot apex) and leaves (the leaflet subtended on the fourth or fifth node from the shoot apex) were fixed in a FAA (formalin/glacial acetic/70% ethanol in the ratio of 0.5 0.5 9.0) and dehydrated through the gradual ethanol series and t-butanol.
Physical examination revealed a pansystolic murmur over the apex and left lower sternal border.
Functional images were obtained with a single-shot steady-state free precession (SSFP) cine sequence covering the left ventricular myocardium from apex to base in 6-mm-thick short-axis slices with 4-mm gaps.
Functional MR-imaging was obtained between the rest and stress PET-scans with a FIESTA (true FISP) cine sequence covering the left ventricular myocardium from apex to base in 8-mm-thick short-axis slices with 2.0 mm gap.
Fresh leaves (second from apex) of three replicates were sampled and frozen in liquid nitrogen from control plants (well watered, ψ w = -0.5 MPa) and moderately stressed plants (ψ w = -1.5 MPa) for RNA extraction.
Leaf material for trichome harvests or microscopy observations was from apexes (including young leaves up to 1 2 cm) from plants aged 2 to 4 months.
Microscopically, left ventricles were cut into 5 μm transversal slices from apex to base.
Left ventricular tissues were equally divided into three parts from apex to the base of heart; the apex, the middle, the base of heart.
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