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Firstly all of the unique sequence counts were aligned to the known pre-miRNA sequences (miRBase v12, September 2008).

Using miRanalyzer, all of the unique sequence counts were aligned to the known pre-miRNA sequences (miRBase v12, September 2008).

The unique sequences from all seabass libraries were submitted to BLASTX similarity searches [ 20] against the zebrafish, tetraodon, stickleback, medaka and human predicted proteomes (downloadable from ).

The consensus sequence for the intron region of M1 mRNA was deduced from all available unique sequences from the NCBI Influenza Virus Resource.

However, this still left 1,253 sequences, including 76 of the unique sequences that had "hits" to proteins from all the other insects tested but not Apis.

For AhLOX7 and AhPLD, the unique sequences obtained from amplicon sequencing were extracted as references.

All identical sequences were compiled to generate a data set of the unique sequences with associated read counts.

The unique sequences were also compared with the 44 Bupleurum protein-encoded sequences from GenBank.

That we obtained a unique YP3 sequence further excludes the possibility of contamination, since this sequence differs from all the currently known sequences.

Sequencing of the top 12 performing clones showed that all had unique sequences (Table 1).

All unigenes were longer than 200 bp and 11.34% (12,309) of them were longer than 1,000 bp.> To estimate the representation of unigenes in the collection, all unique sequences generated from different assemblages were subjected to a BLAST comparison to compare EST collections from a variety of Populus species.