Urine samples (from all populations) were positive for (non-specific) carbaryl metabolite (urine 19/78, faeces 9/99), organophosphate metabolites (urine 103/135, faeces 47/111) and pirimicarb metabolite (urine 72/175, faeces 45/135).
To confirm that the sampled plants were tetraploids, representative plants from all populations (altogether 54 individuals, Table 1) were analysed by flow cytometry to obtain relative nuclear DNA amounts (see Supporting Information Methods S1 for further details on how the flow cytometry analyses were performed).
Cells from all populations were harvested by centrifugation, suspended in 0.9 % NaCl and sonicated for 5 s at 40%% amplitude.
The priest Juan Nepomuceno Solá then proposed that the Cabildo should receive the provisional command, until the formation of a governing junta made up of representatives from all populations of the Viceroyalty.
In all surveys data from all populations were recorded within one week interval.
By the end of June, practically all plants from all populations had shed seed.
Shorter length categories were not tested because all of the individuals from all populations have ROH shorter than this.
The Australian population is significantly different from all populations except the population of Huaraki Gulf, New Zealand.
Thus, removal of non-native species at one location may boost a local subpopulation's viability, but bycatch would continue to drive the species toward extinction by killing older individuals from all populations.
The average annual growth rate determined from all stems collected from all populations was 0.96 mm per year, providing a minimum age estimate of approximately 13,000 years this is our most realistic estimate of the age of the clone, since it takes into account a wide range of growth conditions.
Seeds from all populations were exposed to two conditions.
