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The participants were instructed to go to bed, sleep as normal and write down lights-off and lights-on time from a synchronized watch.
Mature schizonts were isolated from a synchronized culture using 60% Percoll solution (GE Healthcare).
For immunoblots, parasite proteins from a synchronized culture were separated on 7.5% (Bio-Rad Laboratories) or 10% SDS/PAGE and transferred on to nitrocellulose membranes (Schleicher & Schuell).
Animals derived from a synchronized 4-hr egglay were grown at 15° until the L4 larval stage and then shifted to 20°.
(B ) Changes observed in PLM neuronal morphology in wild-type and ptrn-1 (js1286 ) mutants from a synchronized population of animals analyzed at L1 (10 hr) and L4 (36 hr) stages.
For all stress assays, animals derived from a synchronized 4-hr egglay were grown at 15° until the late L3 larval stage and then shifted to 20° and grown for an additional 12 hr until the L4 larval stage.
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Created as a trio effort between the Copenhagen Philharmonic, media design group Makropol, and the Canada-based Helios Design Labs, the World Online Orchestra aims to do just that by incorporating musicians from around the world in a synchronized performance e-space that doesn't even require them to be on the same continent.
In contrast to homogeneous or randomly coupled networks, the transition from a partially synchronized to a fully synchronized phase was found to be less probable due to the role of possible delay in transmission in the resting brain network.
The first time course comprised eleven samples harvested over 56 h from a tightly synchronized P. falciparum 3D7 parasite population: 6, 14, 20, 24, 28, 32, 36, 40, 44, 48, and 56 h post-infection (hpi).
The button itself does not actually trigger the drop; that is done from a control room, synchronized using a government time signal.
WW collection from carefully synchronized worms is a useful method for determining developmental stage-dependent changes but places the worms in an environment without food or accumulated pheromones which could influence the release of ascarosides.
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