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Four different sequences of each class IIα and β MHCII were obtained from a single fish, apparently not derived from a single locus.
We obtained full reconstructions of three rescued swr ears from two fish and two partial reconstructions from a single fish.
Otoliths were taken for age determination from a single fish from each net and a sample of gill tissue was taken for genetic analysis from these and preserved in 96% ethanol.
(A ) A histogram of the IPIs recorded over 20 min from a single fish with spiking receptors (P. microphthalmus ).
Digestions of 1 mg of DNA from a single fish were performed for each enzyme as suggested by the manufacturer (New England Bio Labs).
DNA samples used by the BROAD institute to determine the genomic sequences were for each species extracted from a single fish with 2 N chromosomes.
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To date, several protocols have been developed to achieve clear and identifiable metaphase chromosome spreads from larvae of a single fish species.
The fact that the OR history stretches back to fish was made by [ 6] who claimed that 85%90%% of frog, chicken, mouse and human OR repertoires was constructed from duplication of a single fish OR of group γ, Dr3OR5.4.
Remaining tissue from dissected organs of a single fish were pooled and stored at −80°C for cell culture.
Cells from the pronephros of a single fish were deposited on a Ficoll solution (Lymphocyte separation medium [ d 1.077]; Eurobio, Les Ulis, France) and centrifuged 10 min at 900 G.
A single fish from each group (excluding MPEG) was examined using energy dispersive X-ray analysis (EDX) to localise cadmium, however, cadmium could not be detected in whole body sections.
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