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Genome sequencing of clones from a genomic cosmid library of PDP and in silico selection of the surface exposed proteins were the initial steps in vaccine candidate identification.
Briefly, plastid genome containing clones were identified from a genomic bacterial artificial chromosome (BAC) library that had been constructed for Liriodendron [ 74].
Here, we investigate this question from a genomic perspective: how good a proxy for the ancestral chordate genome is the available amphioxus genome?
From a genomic perspective, the degradome provides a relatively simple representative subset of the coding genome of a species.
The oligonucleotide primers, assigned as 261F and 261R, were designed from a genomic clone of MBV.
From a genomic perspective, why shouldn't the genes and proteins that miraculously combine to create a genius make one who likes to play with DNA?
Ordering genetic markers or clones from a genomic library into a physical map is a central problem in genetics.
These sequence data were used to design PCR primers to isolate the gene encoding BGN6.1 from a genomic DNA library.
The wild-type phenotype of this mutant was restored by one recombinant plasmid (pOPG361) selected from a genomic library of X. campestris pv.
From a genomic DNA library of Trypanosoma cruzi, we have isolated a gene that encodes a polypeptide containing a highly conserved AdoHcy hydrolase consensus sequence.
Next, we used HBV produced by HepDE19 cells a stable HBV-producing cell line that produces HBV from a genomic locus16 to screen for clones susceptible to HBV infection.
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