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MPSS provides a comprehensive assessment of gene expression by generating short sequence tags, each 20 bp long, produced from a defined position for each transcript.
Each tag is derived from a defined position within a transcript.
The major advantage of HT-SuperSAGE over other similar techniques is that 26-bp tag sequences can be isolated from each transcript, which is so far the longest tag sequence obtained from a defined position of transcripts.
Both methods determine the sequence of short tags derived from a defined position at the 3' regions of expressed mRNAs in a cell.
Tag-seq methods generate one tag per transcript, mostly from a defined position, thereby avoiding transcript length biases and length-based data transformation for differential expression analysis.
Both SAGE and MPSS produce short (10 22 nucleotide) sequence tags that are derived from a defined position in a mRNA molecule.
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As anticipated, forward reads aligned at a defined position from the 3' end depending on the sonication parameters (see Additional file 1, Figure S16A).
EXPRSS generates one tag per transcript at a relatively defined position from the 3′ end of a gene, ensuring no length-based data transformation and enabling straightforward statistical analysis.
Clarke has also changed Jones' role, with the player switching from being a winger to having a less defined position.
It's a nebulously defined position, yet a critical one.
We have developed EXpression Profiling through Randomly Sheared cDNA tag Sequencing (EXPRSS) that employs acoustic waves to randomly shear cDNA and generate sequence tags at a relatively defined position (~150-200 bp) from the 3′ end of each mRNA.
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