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As detected by fluorescent in situ hybridization, transcripts from a construct with the tRNA promoter alone localized strictly to the cytoplasm, whereas transcripts from dual U6/tRNA promoter were present in both the cytoplasm and the nucleus.
Clearly, comparing groups within and across countries on selected variables is one meaningful way to gain some understanding of how performance is distributed and connected to outcomes of interest, but this approach doesn't help explain what is being assessed from a construct point of view.
Consistent with our results, Young et al. expressed HIC from a construct that contained HIC-ORF only or from a construct that contained the HIC-ORF and the 3'-UTR (both lacking the HIC 5'-UTR), and detected a much lower HIC protein level when the HIC construct included the 3'-UTR [33].
To determine whether the PD1 promoter contains all the regulatory elements necessary for normal in vivo expression, transgenic mice were generated from a construct containing the 1 Kb PD1 promoter segment ligated to a human CD2 reporter [29].
To show that the VIIth nerve defect is directly due to targeting of HoxB1a by miR-10, we rescued the miR-10 overexpression by co-injecting 5pg HoxB1a RNA from a construct that does not contain any of the target sites.
To first determine the localization of Lsc1p, and also to determine whether modulation of cyclin localization might play a regulatory role in controlling the activity of the constitutively nuclear, Lsk1p, we examined the localization of Lsc1-GFP fusion proteins expressed from a construct integrated at the lsc1 locus and under the control of the native lsc1 promoter (see Materials and Methods).
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Analyses of the six transcriptomes obtained result in 12,219 genes, recovered from a constructed reference transcriptome.
Two data sets are from natural tundra wetlands in Arctic Canada; one is from a constructed wetland in Tanzania.
The dsRNA, which was produced either from a constructed plasmid or from an in vitro transcription reaction, was capable of down-regulating the expression of TgUPRT.
For this research, the low-end nitrogen removal rate and a moderate value estimate of $36.34/kg for nitrogen removal from a constructed wetland was used.
Furthermore, ionospheric delay corrections from a constructed model using GNSS data are also included as pseudo-observations for a better solution.
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