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Exact(3)
Equation 1 defines the detection limit (y), which indicates whether a sample contains a certain Stx based on the average (y B) and standard deviation (SB) of the signal from a blank control (signal of samples without Stx).
After assay validation as described above, cut-off values were set at 2.1, which was two times the average absorbance readings of duplicate negative control sera after subtraction of background readings from a blank control well.
Sequencing produced approximately 48 M total reads from a blank control sample, 56 M total reads from a sample of 0.1% DMSO vehicle, 42 M total reads from a sample of 5 μg/L BDE47, and 38 M total reads from sample of 500 μg/L BDE47.
Similar(57)
Finally, the amount of substrate transported into the vesicles was calculated after subtracting the radioactivity of a blank control from that of the corresponding sample.
These include prior treatment of work surfaces with DNA-destroying agents, use of dedicated pipettes and other instruments, performing DNA extraction and WGA in a restricted chamber with a UV lamp, and the use of a blank control from each tissue section in subsequent WGA and PCR reactions.
IgG pre-incubated with 100-fold excess HBc antibody was used as a negative control, and immunoprecipitation of hepatocyte cells from healthy individuals was used as a blank control.
In all, 10 concentrations of MT201 ranging from 95 to 50 000 ng ml−1 final, plus a blank control, were used.
A blank control group without medication was used as control.
An empty vector was used as a blank control.
A cell control (without LEO) and a blank control (without cells) were performed using untreated coupons.
Water was used as a blank control.
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