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Research in the Sive lab emphasizes craniofacial and brain disorders, using the accessible frog and zebrafish models.
First, there is the yet uncharacterized domain CD2, conserved from mammals to chicken, frog and zebrafish (Suppl. Fig. S1).
Consistent with the presence of the β-catenin-binding domain, immunofluorescence studies also revealed co-localization of Xin with β-catenin in chicken, frog and zebrafish hearts.
Similar to mouse Xins, chicken, frog and zebrafish Xins also co-localized with β-catenin to structures that appear to be the intercalated disc.
To determine the antibody specificity, Western blot analysis was first performed on protein extracts prepared from mouse, turkey, chicken, frog, and zebrafish hearts (Fig. 4).
Unfortunately, we could not determine the colcalization of Xin with gap junction components, since the anti-connexin43 antibody that we used did not cross react with connexin 43 from chicken, frog and zebrafish hearts.
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The research will be guided in part by findings in animal models, including mice, frogs, and zebrafish, he says.
Similarly, in mice, frogs, and zebrafish, amino acid identities of the Apaf-1 interface interacting with cytochrome c were higher than those of the WD40 repeat region.
Consistent with these results, our survey of EST databases in NCBI reveals the absence of Pax2/5/8 genes in the heart transcriptomes of human, mouse, frogs and zebrafish.
Mice, frogs and zebrafish that are defective for Van Gogh-like 2 (Vangl2), the vertebrate ortholog of Drosophila Stbm develop NTDs (Copp et al., 2003).
The phenotypic effect of Lsh depletion in frogs and zebrafish is not associated with loss of any particular germ layer or organ, which dovetails with the range of phenotypes observed in DDM1−/− and antisense MET1 plants [ 53, 54].
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