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Outside the kitchen, to stew is to fret, to be vexed and worried, to fall victim to your own actions.
This enables the distinction between optical mixing of two species and FRET to be made.
For example, one can combine changes in lifetimes, rotational correlation times and spectral information (like FRET) to be more precise in the selection.
Hybridization of r22-Alexa and c22- b-PPO incorporated in the fluorescent N-Rh-PE/DPhyPC liposomes brought the donor and acceptor close enough to each other at the surface, for an efficient FRET to be observed.
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We are currently trying to use heme as a FRET acceptor and a blue fluorescent protein as a FRET donor in fluorescence lifetime measurements (FLIM-FRET) to be able to circumvent fusion of a fluorescent protein to the amino-terminus of the β1 subunit.
Numbers, rather than letters, indicated which fret was to be stopped.
The intonation tuning can be when the pitches of the first fret to seventh fret notes are in tune to a chromatic electronic tuner reference pitches.
"Pretty damn soon," she frets to Evans, "there's gonna be no corn in Egypt, baby".
Furthermore, potential effects of protein conformation changes on FRET need to be considered.
Don't fret -- there seems to be plenty left on Apple's website.
If she eats it great, but if she scurries back under cover do not fret, it is to be expected.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com