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Ribonucleic acid samples were prepared freshly and subjected to quality analysis.
Small aliquots of the freshly prepared PRP were subjected to blood cell count.
The roots for assay were freshly sampled and immediately subjected to enzyme assay after processing.
The freshly harvested broccoli were subjected to MV storage (70 ± 5 kPa) and atmospheric pressure storage at 3 ± 1 °C for 49 d; The relationship between broccoli chlorophyll content and its surface color parameters (-a/b value) was studied to build broccoli chlorophyll content prediction model based on the color parameter changes under MV conditions.
Freshly isolated cells were subjected to phenotypic analysis or kept at −80°C until use.
Two days later, the freshly infected cells were subjected to G418 selection.
Freshly prepared extracts were subjected to a preliminary phytochemical screening for various constituents.
In order to examine the apoptotic effects of TRAIL and FASL in an in vitro system of human colorectal carcinogenesis, we subjected the freshly isolated primary colon cultures PAP60 and MIH55 and the established colon cells lines Caco-2, HT29 and DLD-1 to TRAIL and FASL treatment.
Single cell suspensions from freshly thawed pellets were subjected to basal and luminal population discrimination by FACS-sorting using an allophycocyanin-conjugated rat antibody to human CD49f and a phycoerythrin-conjugated mouse antibody to human EpCam, respectively (Eirew et al, 2008).
A comparison between the freshly calcined sample and samples subjected to simulated regeneration cycles was demonstrated.
Therefore, freshly prepared NPΔ451 739 protein was subjected to make a more detailed time-lapse EM analysis to capture the various conformers at different stages of NLC assembly.
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