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Upon thaw, the pre-labeled cells displayed viability and label retention comparable to freshly labeled cells, responded to ADCC mediated by both peripheral blood mononuclear cells and engineered natural killer cells, performed stably for at least 3 years and provided favorable precision and accuracy to ADCC assays.
On the way to our rooms on the third floor, we passed a door with a card stuck on it freshly labeled in neat calligraphy, "HRH Prince Michael of Kent".
The freshly labeled Art Basel Hong Kong recently announced its 2013 lineup, featuring over 48 galleries who have never shown in the Asian city before.
This is a sophisticated approach because it requires frequent preparation of freshly labeled substrate during the experiment [19], [29], [30].
Tumor cell extravasation assays: A total of 4 × 10 TCs freshly labeled with CellTracker™ Red CMPTX (Invitrogen C34552: ex/em = 577/602 nm) were seeded into each insert.
The stability in serum was analyzed by mixing murine serum (200 μL) with 10 μL freshly labeled 99mTc-ZIGF1R:4551-GGGC.
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This is usually an unlabeled peptide nominally added in excess to the reaction to prevent the rebinding of freshly dissociated labeled peptide.
Freshly purified labeled test and control cDNA were combined prior to incubation with hybridization solution (Final concentrations: 3× SSC, 24 mM HEPES (pH 7.0), 0.225% SDS) at 95°C for 2 minutes.
In addition, the in vivo survival of freshly isolated, CFSE labeled Tregs demonstrated these cells had very poor survival relative to expanded cells decreasing the potential biologic impact of transferred freshly isolated cells.
To visualize the entry of freshly isolated and labeled T cells into already inflamed joints, some SCID mice were injected first with unlabeled donor cells.
Uninfected and infected macrophages and freshly prepared monocytes labeled with red (PKH26) or green (PKH67) dye (Sigma-Aldrich, St Louis, MO) mixed at a 1 5 ratio were seeded onto the contracted tissue along with epithelial cells at a density of 10 cells per model.
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