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Prior to the Neutralization assay, 500 µL antisera (diluted 1∶50, and pre-absorbed with induced yeast cells carrying the control vector pCTCON-2) were mixed with freshly induced yeast cells (1×108) displaying specific antigenic peptides on surface.
After incubation at 4°C for 12 h, the mixtures were centrifuged at 8,000 g for 1 min and supernatants mixed with another batch of freshly induced yeast cells.
3 IFM on a freshly induced blister is now recommended as the primary method for diagnosing EB, because IFM can provide considerable insight into the precise level of tissue cleavage and into the relative expression and distribution of components of the skin basement membrane zone and epidermal antigens.
To label cells for FACS, 1×107 (For yeast EBY1001 cells, OD600≈1×107 cells/mL) freshly induced library cells were pelleted at 8,000 g for 1 min in a 1.5 mL microcentrifuge tube and washed two times with 1 mL of 1×Phosphate Buffered Saline (PBS).
Extensive analysis of the DNA damage response revealed increased basal levels of DNA damage in fibroblasts and increased damage at telomeres in lymphocytes but crucially, the response to freshly induced DNA damage was not increased.
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Addition of feedstock was described to prolong the phase in which enzyme production is most active by freshly inducing both biomass growth and enzyme expression [ 30, 32].
The relaxation of these freshly induced ions towards an equilibrium distribution occurs due to Coulomb interactions and wave particle interactions with the background turbulence.
On a daily basis, sponges were pulse-fed for 4.5 hours with freshly harvested induced, actin dsRNA-expressing HT115 DE3)/L4440-actb bacteria.
Electrophoretic assays of in vitro DNA strand breakages showed that freshly fractured silica induced DNA strand breakage, which was inhibited by catalase and enhanced by H2O2.
We found that CM, but not fresh medium, induced CREB phosphorylation on Ser-108/111/114 in freshly plated HEK 293T cells (Fig. 4B).
Lesions were induced in freshly excised bovine liver samples.
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