Colonic tissues from the proximal and distal portion of the colon were freshly isolated and embedded in paraffin wax after fixation with 10% neutral buffered formalin.
Colonic tissues from the proximal and distal portion of the colon were freshly isolated and embedded in paraffin wax after fixation with 10% neutral 10 buffered formalin.
For cryosectioning, embryos fixed with 4% PFA overnight or freshly harvested were embedded in Tissue-Tek OCT compound (Sakura Finetek, USA) and 14-μm sections were generated.
The basic research related to this clinical trial showed that freshly isolated chondrocytes embedded in type I collagen gel could proliferate, synthesize ECM, and maintain their chondrocytic phenotype for up to 4 weeks (Chaipinyo et al. 2004).
As Hoover explains in the journal, the filaments "found embedded in freshly fractured interior surfaces of [certain meteorites] are interpreted as the fossilized remains of prokaryotic microorganisms that grew in liquid regimes on the parent body of the meteorites before they entered the Earth's atmosphere".
For IF experiments on cardiac tissue sections, freshly isolated hearts were embedded in Optimal Cutting Temperature compound (OCT) and cut in 5 μm sections.
For histopathological and immunohistochemical examinations, the collected tissue samples were fixed in 4%, freshly prepared, buffered paraformaldehyde, embedded in paraffin according to routine histological procedures and sectioned at the thickness of 5 μm.
Freshly dissected female brains (4 5 days old) were embedded in cryo-embedding medium (Tisse-Tek OCT, Fisher Scientific, Pittsburgh, PA).
Freshly dissected pituitaries were fixed in 4% paraformaldehyde, 0.2% glutaraldehyde in 0.12 M cacodylate buffer (pH 7.4, 2 h on ice), postfixed in 1% osmiumtetroxide (1 h) and embedded in EMBed-812 (Science Services).
