Freshly sorted EC defined as Sca-1+, CD31+, CD45− along with Sca-1−, CD31−, CD45− control cells were harvested from 2 MO C57BL6 mice and also transplanted via direct injection into the TA.
Therefore freshly sorted muscle EC defined as Sca-1+, CD31+ and CD45− cells are angiogenic, mature EC.
When cultured in collagen I gels using defined conditions, freshly isolated basal HMECs give rise to complex branched multicellular structures.
To collect individuals of defined age, freshly eclosed animals were captured, marked individually with a plastic tag on their thoraces and put back into the colony.
To obtain cultures of a defined age, mycelium from freshly germinated ascospores was placed on one side of a Petri dish containing PASM.
Washout of a material is defined as the tendency of freshly prepared cement paste to disintegrate upon early contact with fluids such as blood or other fluids.
Natural ageing is defined as handling and storage of freshly harvested grain for several years under storage condition to achieve suitable flour quality after milling.
The activity of freshly prepared beads in the first run was defined as 100%%.
On the next day freshly laid Manduca eggs were counted in a defined area on the plant, approx 30 cm around the scented cotton swabs and afterwards removed.
