Sentence examples for freshly constituted from inspiring English sources

Cells (1×10) were re-suspended in 4 ml of freshly constituted permeabilising solution (0.35% Triton X-100, 0.1 m M PMSF in nucleus buffer), and mixed by rotation at 4°C for 20 min.

The substrate was prepared in freshly constituted Base Reaction Buffer [comprising 20 mmol/L HEPES, pH 7.5 ; 10 mmol/LMgCl2; 1 mmol/L EGTA; 0.02% BRIJ-35; 0.02 mg/ml BSA; 0.1 mmol/L Na3VO4; 2 mmol/L dithiothreitol (DTT); and1% DMSO].

unc-4 GFP unc-4 GFPrarely observed in freshly dissocellsd preparetions but constitute about 2% of all cells afterarelyy in culture.

Those constituted the positives.

CNBr-activated Sepharose 4B was derivatized with 6-aminohexanoic acid to constitute a spacer arm prior to coupling with freshly prepared 4-aminophenylarsine oxide (high solubility in DMSO) via carbodiimide.

What constitutes male, what constitutes female?

It constitutes moral abdication.

What constitutes not trying?

What constitutes success?

Did that constitute abuse?

First, what constitutes "important"?