Sentence examples for freshly complemented from inspiring English sources

For ubiquitination assays, cells were harvested 48 h post-transfection by sonication in Ub-lysis buffer (50 mM HEPES (pH 7.8), 200 mM NaCl, 5 mM EDTA, 1% NP40, 5% glycerol, freshly complemented with 1 mM DTT, protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail II (Sigma), and 250 ng/ml ubiquitin-aldehyde (Sigma)).

At positive back-gate voltage, the significantly suppressed electron injection from the freshly absorbed NH3 is complemented by the electron extraction from graphene to the original p-type doping agent.

Subsequently 2 µL of rabbit complement mixture (1 mL freshly used complement, supplemented with 15 µL mixture of 0.03% acridine orange and 0.1% ethidium bromide (Ampliqon)) was added to the cells, mixed and incubated at rt in the dark for 60 min. As positive control we stained the cells either with a pool of HLA-A antibodies or PBMCs expressing the corresponding HLA alleles.

Then 3%CD599 antiserum and 1.6% freshly thawed complement (v/v) were added to each dish.

The cultures were incubated for 2 hr to allow for cell attachment, after which 0.2%CD599 antiserum and 1.5% (vol/vol) freshly thawed complement were added to each dish.

Osteogenic medium consisted of alpha-MEM medium complemented by 10% FBS, 50 µg/ml freshly prepared ascorbic acid (Sigma, Saint-Quentin, Fallavier, France), 10 mM sodium-beta-glycerophosphate (Sigma) and 300 ng/ml recombinant human BMP4 (RnD systems, Lille, France).

The sheep complemented it".

Our job complemented theirs.

"We complemented each other.

The drink comes in a glass tube with small holes along the top into which edible flowers and freshly foraged sprigs of pine are placed, releasing an aroma to complement the lemony acqua di cedro liqueur in the drink.

Complementing the familiar concept of harmonics (pitches drawn from the overtones of a given fundamental), Ms. Kimura has named her freshly discovered sonorities "subharmonics".