Exact(1)
Fresh transformations of each construct were made and plated on to 90 mm 2 × TY 1.5% (w/v) agar plates containing 100 μg/ml ampicillin.
Similar(59)
The result is an ingeniously fresh transformation that sounds like crossing a 90s diva with Floating Points and throwing in a bunch of confetti.
Even though the cryopreservation of E. coli might lead to a loss of viability [ 12, 13] and product yield [ 13], often cryocultures are established from the preculture-MTP, as it can be impractical to prepare a fresh transformation mix for each expression study.
But this is a fresh, intelligent transformation, terrifically involving all the way through; it has responded to the human drama and found a persuasive anti-hero in Clough.
The art of Le Parc remains fresh despite the many transformations, in both aesthetics and techniques, that took place in the years to follow.
Tips From a Teenage Decorator USE FLOWERS "Never underestimate the beauty and transformation that fresh flowers can add to a room," Sam Allen said.
As Emmott shows in powerful detail, it is human expansion that lies behind the conquest of our dependency on oil, coal and gas; the industrial-scale use of chemical pesticides, herbicides and fertilisers; battery farming; the transformation of fresh water into a depleting resource and the mass extinction of other life forms that is under way.
The collection doesn't come close to the vast Islamic holdings of the British Museum or the V&A, nor the Louvre or the Metropolitan Museum of Art, But as museum director, Henry Kim, fresh from leading the transformation of Oxford's Ashmolean Museum, puts it: "It is a connoisseur's collection.
Fresh off his colorful transformation of the Spanish city Asturias' Church of Santa Barbera into the Kaos Temple, Okuda returns to the altar by decking out the Moroccan chapel in his signature multicolored geometric characters.
Interactions were then confirmed through the transformation of fresh AH109 yeast with sequence-verified pGADT7 vectors, and with mating and β-galactosidase assays performed as described above.
Each transformation is a fresh reminder that he can't be fixed in either sense: made better or fully apprehended.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com