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Fresh supplements were added at day 3 or 4 and both adherent and non-adherent cells were kept when replating the cells at day 7. Experiments were started after 10 14 days of culture in stem cell media.
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Both immobilized endoxylanase were successfully reused up to 4 hydrolysis cycles with a fresh supplement of Cellic® C-Tec2 each time.
The content-scent has the property similar to the natural scent that can spread over air, mix with other scents, decay with distance and time, and strengthen with fresh supplement.
Medium was replaced by fresh supplemented medium.
Cells were then collected via centrifugation and resuspended with fresh supplemented M9 media.
After removal of medium from the culture chambers, 10 000 sporozoites were added in 100 µl of fresh supplemented medium.
The cells were washed with medium containing 10% FCS and grown in fresh supplemented endothelial cell growth medium.
Following BMMC culture as described above, nonadherent cells were removed from cultures at specific intervals and were replaced by 1000 µl of fresh supplemented IMDM.
Culture medium was partially replaced every 3 4 days with fresh supplemented Neurobasal medium.
Cultures were fed biweekly by replacing 50% of the medium with fresh supplement.
Expand with fresh supplemented DMEM when needed (if the medium changes color from red to orange/dark yellow).
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