Sentence examples for fresh stained from inspiring English sources
Electron-dense "spherules" ranging in size from 200 800 Å were also identified within mitochondria of the chondrocytes (cartilage cells) in the proliferative and hypertrophic zones of fresh, stained, tibial epiphyseal plates and occipital bones of mice and rats.
The Bissell was not able to remove dried stains, but it removed fresh stains efficiently.
Fixed cells were stained with fresh staining solution for SA-β-gal activity at 37°C overnight, and counted for positivity (N > 300).
A fresh staining solution should be used.
If this happens, fresh Stain buffer should be added or the original volume you incubate in should be increased.
After adding antibodies, the cells were incubated on ice for 45 min, then washed with 2.5 ml fresh staining medium and pelleted (220g for 4 min at 4°C) and resuspended in secondary antibodies.
Digested SVZ pieces were centrifuged (220× g, 4 min, 4°C) then fresh staining medium was added and the pieces were triturated in 300 μl by gently drawing into a P1000 pipetman and expelling 25 times without forming bubbles.
This should work on fresh stains.
Fresh stains are easier to remove than old ones.
Fresh stains should be soaked and agitated in cold water before washing.
Like I said, any fresh stain, removed or not, needs to be washed right away.
