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Media was removed each well and replaced with fresh media containing 1 mg/mL MTT.
Particles were resuspended in 10 mL of fresh media.
Cells were resuspended in 1 mL fresh media and used for DNA extraction.
At a regular time interval, the liquid media were replaced by aliquots of fresh media.
Whole media were replaced with fresh media at specific time intervals.
The chamber slide was incubated at 37 °C overnight for attachment and fresh media was added.
Then, the cell media were discarded and replaced with fresh media.
Once cells reached late exponential phase they were transferred to a fresh media amended with furfural.
At every 4 weeks intervals, the cultures were sub-cultured in their respective fresh media.
Fresh media containing inhibitors were added when the cell culture was split.
After that, media were discarded and then replaced with 100 μl of fresh media.
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