Sentence examples for fresh isolation from inspiring English sources

After 10 min, the CD4-enriched cell suspension was then transferred into a new tube and the magnetic separation process repeated for 5 min with fresh Isolation Cocktail and RapidSpheres.

Cells were then resuspended in fresh isolation solution and imaged using a laser scanning confocal microscope (Leica, Germany) with 488 nm excitation light and detection at > 505 nm.

The fresh isolation of the pathogen together with the observed high seroprevalence demonstrates the presence and frequent exposure of cattle in the area to the pathogen.

Cardiomyocytes were agitated gently, filtered through a nylon mesh, and stored in fresh isolation solution at room temperature for up to 8 h.

Once viable cardiomyocytes were observed, the suspension was removed from the shaker, left to settle, and resuspended in fresh isolation solution.

When this protocol was used, the tissue did not require the subsequent shaking step in the water bath, and viable cells were achieved after 20 min of enzyme perfusion followed by gentle agitation of the tissue in fresh isolation solution.

These measurements were made on eight separate days, on six to eight fresh isolations, with two to four PCRs per isolation (Table  1).

The difference between the recovery of viable SSEA4+ cells isolated before and after cryopreservation of cells was not statistically significant (P = 0.1484), as was the difference between fresh tissue isolation and frozen tissue isolation (P = 0.5432).

When fresh cell isolations were compared to frozen cells, we saw no difference in the morphology, development, or viability over time of these neurons.

The EBR was removed and the ovaries were flash-frozen in liquid nitrogen or used fresh for RNA isolation.

The complete treatment involves three cycles 2 weeks apart (weeks 0, 2, and 4), with each cycle comprising fresh leukapheresis, cell isolation and culture with PA2024, and reinfusion.