Got it; now let's have that fresh, Gen-X take on this tired conceit.
After infection in 5% CO2 for 1 h at 37°C, the infected monolayers were gently washed three times with 1 ml HBSS to remove noninvasive bacteria, and incubated for 1 h in fresh medium containing gentamicin (GEN; 100 μg/ml) to kill the extracellular bacteria [ 2, 7- 9].
The next day, cells were treated with free GEN, GCN, and FGCN in 100 μl of fresh media and incubated for 24 h.
Next day, cells were treated with free GEN, GCN, and FGCN in 100 μl of fresh media and incubated for 24 h.
In late September, Lincoln met with Gen. Oliver Otis Howard, fresh off a victory at Gettysburg and on his way to reinforce Rosecrans in Tennessee.
Finally, over a month after the outbreak of the Sioux uprising, Lincoln responded, assigning Gen. John Pope, fresh from his defeat at Bull Run, the task of ending the uprising.
Programs such as Nolcha Fashion Week and Gen Art's Fresh Faces in Fashion are organizations that help new designers get started.
Even as fresh hires, the hyper-connected Gen Z-ers will have strong networks available to them.
Fresh off stunning victories at Fredericksburg and Chancellorsville, Gen. Robert E. Lee now boldly committed his great Army of Northern Virginia to a major invasion of the enemy's heartland.
