For the immunohistochemical studies, we recorded antibody used, type of specimen (fresh fixed, fresh unfixed, fixed archival) and the criterion used to define abnormal accumulation of p53.
13 MicroRNAs (miRNAs) are more resistant to degradation than messenger RNA (mRNA) and can be detected in fresh, fixed, or frozen tissue samples and in the peripheral blood.
The mixed linear model, implemented in SPSS version 20.0 (IBM SPSS Inc., Chicago, IL), was used to analyze differences in ISQs as a function of implants and femoral bone treatment (fresh, fixed, or decalcified).
Total RNA was extracted from fresh, fixed and fixed/sorted cells according to the Tri Reagent protocol, with the addition of Glycoblue during the isopropanol incubation for RNA isolation.
Most findings presented here derive from PCR-based studies, the comparability of which depends on the quality of the biological specimens (bile, stone, or tissue biopsies; fresh, fixed, or frozen), the strategy chosen for Helicobacter detection, and potential problems such as contamination and the presence of Taq polymerase inhibitors.
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Bisected brains were immersion fixed in 0.1 M phosphate buffer pH 7.4 containing 4% PFA and 2.5% glutaraldehyde for 2 hours, incubated in fresh fix solution for 2 days, and stored in 10 mM phosphate buffer at +4°C until processed further.
There is something appealing about the idea of a fresh start fixing things; a recent Politico story suggested that the new Speaker and the White House might even be able to collaborate in a way that President Obama and former Speaker John Boehner could not.
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