Sentence examples for fresh fixation from inspiring English sources

But now Mount has combined these with a fresh fixation: the 60s.

The buffers were removed and the cells were incubated in fresh fixation solution for 2.5 h at 4 °C.

Whole jejunum and colon tissues were fixed in a fresh fixation buffer (3% glutaraldehyde and 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4) for several days at room temperature.

The main changes included trimming fresh specimens, fixation during the process schedule overnight, removal of unnecessary steps, laboratory reorganization, team-based reporting and extending the working day.

Human pigmented lesions were excised and imaged fresh, without fixation or treatment.

Cultures were incubated at 37°C for 72 h, colchicine (final concentration of 0.1 μg/ml) was added for 1 h, and hypotonic treatment (0.075 M KCl at 37°C for 15 min) and four washes were carried out, each with a fresh cold fixation solution (3:1 methanol: acetic acid solution).

Detailed and standardised descriptions are required in particular for immediate procedures, such as harvesting of the brain, sampling from the fresh brain and fixation that can be carried out by training level 0 personnel (Table  1) but in the same vein may pose essential limits to the adjacent work-up, diagnostic yield and accuracy.

After incubation for 4 h, the coverslips were placed in chilled distilled water for 10 min and fixed for 1 h in ice-cold 3 1 ethanol/glacial acetic acid, followed by 10 min re-fixation in the fresh fixative.

We have previously described a novel tissue fixation and processing technique that beside providing adequate histomorphology also preserves high quality HMW RNA, akin to fresh samples [ 19].

At the end of the fixation, a fresh stimulus was presented for 1 -s, followed by a 3 -s fixation.

For peroxidase detection, frozen sections were air-dried for 30 min. prior to fixation in fresh acetone for 15 min. (BDH).