Exact(60)
The cells were continuously cultured in fresh complete medium for 48 h.
The cells were then washed with PBS, and fresh complete medium was added.
After 2 h, wells were rinsed twice to remove unbound RFP-vector and fresh complete medium was replaced.
Cells were then washed twice and resuspended in fresh complete medium.
After various lengths of incubation, cells were washed twice and resuspended in fresh complete medium.
After 24 h, the transfection medium was replaced with fresh complete RPMI medium.
After 4 hours, the medium was changed to a fresh complete culture medium.
Cells were diluted in fresh complete medium and seeded in 96-well plates.
Every two days the medium was removed and 2 ml of fresh complete medium was added.
ASM cells (4×104) were seeded into 12-well culture plates in fresh complete DMEM.
Fresh complete media was added and replaced every three or four days thereafter.
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