Exact(11)
In order to study the effect of PHMB-stabilized Ag NPs on bacterial growth, E. coli strain was grown on an agar plate, and its fresh colony was transferred with the help of a sterilized loop into a shake flask which was then incubated in an incubator shaker for 12 h at 37°C at a shaking speed of 150 rpm under aerobic conditions.
Overnight cultures were started by picking a fresh colony from a TSA blood agar plate (B.
To evaluate the susceptibility of MRSA to NO-np, TSB was inoculated with a fresh colony grown on BHI plates and suspended in 1 mL of medium.
A fresh colony was inoculated into YPD medium and incubated at 30 °C overnight.
Briefly, a fresh colony of S. pneumoniae grown overnight on BAP was scraped and grown in TSB+1% glucose medium [ 32].
We used general linear models to estimate the effects of endophyte treatment (nested within sampling time) on fresh colony mass, number of workers, queen mass, dry garden mass, fresh colony mass per worker (× 1000), and dry garden mass per worker (× 1000).
Similar(49)
Fresh colonies were used for saccharification and fermentation studies.
A starter culture of each strain was inoculated with fresh colonies and incubated for 24 h in Tripticaseine medium.
Independent experiments (biological repeats) were done at different days, with fresh colonies and fresh media.
Spotting assays derived from a pool of three independent fresh colonies.
G. vaginalis 14018 was grown on an HBT plate and fresh colonies were resuspended in lysis buffer (BugBuster, EMD Chemicals, Gibbstown, NJ) with benzonase nuclease.
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