Males also were attracted by fresh blotting paper containing females' draglines, while females do not respond to fresh blotting paper containing males' draglines.
Males also were attracted by fresh blotting paper containing females' draglines, while females do not response to fresh blotting paper containing males' draglines.
Fresh blots were used for each channel of interest, and each blot was reprobed with GAPDH for normalization and to control for variability during sample loading.
I watched as she flushed away the cigarette, rinsed her mouth at the sink, and applied fresh lipstick, blotting it with a Kleenex, which then fluttered into the garbage pail.
Then, non-adherent cells were removed and the adherent cells were recovered and prepared for Western blot (fresh monocytes) or left with only medium (non stimulated monocytes) or stimulated for 48 h with LPS (2.5 μg ml−1; Sigma Chemical Co., Munich, Germany), PHA 2 μg ml−1, human recombinant IL-4 (rIL-4) 100 U ml−1 or GM-CSF 100 U ml−1 (generously donated by Shering Plough, Brinny Co., Ireland).
(B) One-third of the tumors were processed "fresh" for Western blot analysis by directly homogenizing them in 500 μl of radioimmunoprecipitation assay (RIPA) lysis buffer, and the final third of the tumors were freeze-thawed (F/T) by snap-freezing in dry ice and storing them at −80°C for 1 hour.
A laboratory test showed how males of P. labiata minimise the risk of meeting each other, by recognising fresh pieces with blotting paper, some containing their own silk draglines and some containing another male's.
A laboratory test showed how males of P. fimbriata from Queensland minimise the risk of meeting each other, by recognising fresh pieces of blotting paper, some containing their own silk draglines and some containing another male's draglines.
Y-LN carried out extraction of M. grandiflora L. fresh flowers and Western blot.
