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The top 20 most frequent unique transcripts in the cDNA library of the 4-year old P. notoginseng root were shown in Table 2.
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Further, the fraction of unique transcripts recovered is consistent with the fraction previously reported23.
Of all the HEGs, more unique transcripts were expressed over TPM500 at the green stage (46 transcripts) other than yellow and red stages (27 and 8) (Fig. 1c).
After mapping to the human genome (GRCh38) and annotating using only polyadenylated transcripts, the number of unique transcripts could be determined.
The number of unique transcripts for each sample was then identified, and the correlation between replicates calculated.
The Plant Genome Database (http://www.plantgdb.org/) had 77,500 Putative Unique Transcripts (PUT), (http://www.plantgdb.org/download/download.php?dir=/Sequence/ESTcontig/Pinus_taeda/current_version/Pinus_taeda.mRNA.PUT.fasta.bz2).
Wang et al. (2007) identified 162 unique transcripts corresponding to possible salt-related genes in P tenuiflora.
When N transcripts are sequenced, there are s(N) = S[1−P(0|N)] unique transcripts expected to be detected.
Certain genes correspond to multiple transcripts, and in terms of transcripts, our data consists of 98 pairs with 123 unique transcripts.
Using this approach, the full PS dataset was summarized into a 19,871 unique transcripts dataset.
This analysis assigned EC-enrichment of more than two-fold to 178 unique transcripts.
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